HEPBS CAS:161308-36-7 Kumukūʻai Mea Hana

N-(2-Hydroxyethyl)piperazine-N'-(4-butanesulfonic acid) (HEPBS) ʻo ia ka zwitterionic buffer i hoʻohana mau ʻia i ka noiʻi olaola a me ka biochemical.ʻO kāna hopena mua, ʻo ia ke kōkua i ka mālama ʻana i ka pH paʻa i nā hoʻonā, ʻoi loa i loko o ka pae pH physiological (7.2-7.4).

ʻO ka noi nui oHEPBS aia i loko o ka moʻomeheu cell, kahi i hoʻohana ʻia ma ke ʻano he ʻāpana o ka media moʻomeheu e mālama i ka pH o ka hopena.Kōkua ia i ka hāʻawi ʻana i kahi kūlana paʻa no ka ulu ʻana o ke kelepona a pale i nā loli pH hiki ke hoʻopōʻino i nā cell.

HEPBS Hoʻohana ʻia ʻo ia ma ke ʻano he buffering agent i nā haʻawina enzyme, no ka mea hiki iā ia ke hoʻopaʻa i ka pH i ka wā o nā hopena enzymatic.Hoʻohana maʻamau ia i ka hoʻomaʻemaʻe protein a me nā hoʻokolohua enzymatic e hoʻopaʻa i ka hana a me ke kūpaʻa o nā enzymes.

Eia kekahi,HEPBS Hoʻohana ʻia i nā ʻano hana electrophoretic like ʻole, e like me ka electrophoresis gel a me ka electrophoresis capillary, e mālama i ka pH i makemake ʻia a hoʻopaʻa i nā molekala i hoʻopaʻa ʻia e hoʻokaʻawale ʻia.

Ma waho aʻe o kāna mau waiwai buffer,HEPBS Hiki iā ia ke hana ma ke ʻano he mea hoʻonāwaliwali nāwaliwali o kekahi mau metalloproteins a me nā enzymes, e hoʻohana pono ai i nā noi kikoʻī.