HEPES CAS:7365-45-9

He kuleana koʻikoʻi ko HEPES i ka mālama ʻana i kahi kūlana pH paʻa i loko o nā ʻōnaehana moʻomeheu cell, e hōʻoia ana i ke ola a me ka ulu ʻana o nā cell ma ka pale ʻana i nā loli i nā pae pH. He mea nui loa kēia hiki ke hoʻopaʻa ʻana o HEPES i ka mālama ʻana i nā kūlana physiological no ka noiʻi cell a me ka hoʻāʻo ʻana i nā lāʻau lapaʻau, e alakaʻi ana i nā hopena hilinaʻi a hiki ke hana hou ʻia. I loko o ka biology molecular a me ka biochemistry, hoʻohana pinepine ʻia ʻo HEPES i nā hoʻāʻo enzyme e hoʻoponopono pono i nā hopena enzymatic pH-sensitive. ʻO kona hiki ke mālama i kahi pH mau i loko o kahi pae haiki (maʻamau 7.2-7.6) he mea nui ia no ka hōʻoia ʻana i ka pololei a me ka pono o nā kaʻina hana biochemical, e like me ka hoʻomaʻemaʻe protein, ka nānā ʻana i ka nucleic acid, a me nā haʻawina metabolic. Hoʻohana ʻia ʻo HEPES i nā noi electrophoresis, me ka agarose gel electrophoresis a me ka polyacrylamide gel electrophoresis, kahi e kōkua ai i ka hana ʻana i kahi kūlana kūpono no ka hoʻokaʻawale ʻana o ka DNA a me ka protein. Hāʻawi kona mau waiwai buffering i nā kāʻei ʻoi a wehewehe maikaʻi ʻia, e hoʻonui ana i ka hoʻonā a me ka pololei o nā hopena hoʻokolohua. Eia kekahi, loaʻa iā HEPES ka hoʻohana nui ʻana i ka ʻoihana lāʻau lapaʻau no ka hoʻokumu ʻana i nā huahana biological, nā lāʻau pale, a me nā reagents diagnostic. ʻO kona kūlike me nā biomolecules koʻikoʻi a me ka cytotoxicity haʻahaʻa e lilo ia i koho kūpono no ka mālama ʻana i ke kūpaʻa a me ka hana o nā biopharmaceuticals i ka wā o ka hana ʻana a me ka mālama ʻana. Ma keʻano holoʻokoʻa, lawelawe ʻo HEPES ma ke ʻano he mea hoʻopaʻa buffering versatile a pono ʻole me nā noi like ʻole i ka biology cell, ka noiʻi molecular, biochemistry, a me ka hoʻomohala lāʻau lapaʻau, e pāʻani ana i kahi kuleana koʻikoʻi i ka hōʻoia ʻana i ka holomua a me ka hilinaʻi o kahi ākea o nā hoʻokolohua biological a me biochemical.