Iodoacetamide CAS:144-48-9 Kumukūʻai Mea Hana

Hoʻololi ʻana o Cysteine: Hoʻopili ʻo Iodoacetamide me nā hui thiol manuahi i loko o nā koena cysteine, e hana ana i kahi pilina covalent me ka cysteine ​​sulfur. Kōkua kēia hoʻololi i ka nānā ʻana i ka protein ma ke ālai ʻana i nā koena cysteine ​​a me ka pale ʻana i nā hopena i makemake ʻole ʻia a i ʻole ka oxidation.

ʻO ka hoʻoheheʻe ʻana o ka protein: Hoʻohana pinepine ʻia ʻo Iodoacetamide i nā kahe hana hoʻoheheʻe protein no ka nānā ʻana i ka spectrometry mass. Hoʻopili ia me nā koena cysteine ​​​​ma mua o ka hoʻoheheʻe enzymatic, e pale ana i ka hoʻokumu ʻana o ka pilina disulfide makemake ʻole ʻia a mālama i ka protein i kona kūlana i hoʻemi ʻia.

Nā Haʻawina Proteomics: Hoʻohana ʻia ʻo Iodoacetamide i nā hoʻolālā lepili e pili ana i ka proteomics e pili ana i ka spectrometry mass. Ma o kāna hana ʻana me nā koena cysteine, hiki iā ia ke lepili koho a me ka ʻike ʻana i nā peptides i loaʻa ka cysteine.

Nā Haʻawina Kūkulu Protein: Hoʻohana ʻia ʻo Iodoacetamide i nā haʻawina kūkulu e hoʻololi kemika i nā koena cysteine ​​a aʻo i kā lākou hana a me ke komo ʻana i ka pelu ʻana o ka protein, ka nakinaki ʻana, a me nā loli conformational. Hiki nō hoʻi ke lilo i mea pono i nā hoʻokolohua cross-linking e palapala i nā pilina protein-protein.

Hana ʻana o nā Peptides i Lepili ʻia: Hiki ke hoʻohana ʻia ʻo Iodoacetamide e hana i nā peptides i lepili ʻia ma ka hoʻololi ʻana i nā koena cysteine ​​​​me nā hui i lepili ʻia isotopically, e like me deuterium a i ʻole carbon-13. A laila hoʻohana ʻia kēia mau peptides i lepili ʻia i nā noiʻi like ʻole, me ka helu ʻana o ka protein a me nā haʻawina dynamics.