ʻO Iodonitrotetrazolium chloride CAS: 146-68-9 Kumukūʻai Mea Hana

Hoʻohana pinepine ʻia ʻo Iodonitrotetrazolium chloride (INT) e ʻike i ka hana o nā enzymes dehydrogenase i loko o nā ʻōnaehana olaola. Pili kēia mau enzymes i ka metabolism cellular a he kuleana koʻikoʻi ko lākou i nā ala biochemical like ʻole. He mea pono loa ʻo INT i nā assays i hoʻolālā ʻia e loiloi i ke ola o ke kelepona a me ka hana metabolic. Ke hoʻohui ʻia ʻo INT i kahi hāpana hoʻāʻo, ʻo ka hōʻemi ʻana o ka dye e nā enzymes dehydrogenase e hopena i ka hoʻokumu ʻia ʻana o kahi huahana formazan ʻulaʻula. Hiki ke ana ʻia ka ikaika o ke kala ʻulaʻula ma ke ʻano spectrophotometrically, e hāʻawi ana i kahi ana quantitative o ka hana enzyme a me ke ola o ke kelepona. ʻO kēia ka mea e waiwai ai ʻo INT i nā ʻano noi like ʻole, me nā assays proliferation cell, nā assays microbial viability, a me ka loiloi ʻana i nā hopena lāʻau lapaʻau ma ka metabolism cellular. ʻO kona hoʻohana ʻana i nā noiʻi a me nā assays diagnostic ua kōkua i ka hoʻomaopopo maikaʻi ʻana i nā kaʻina hana physiological a me nā pathological ma ka pae cellular.