Leupeptin CAS:24125-16-4

Hoʻohana nui ʻia ʻo Leupeptin i nā keʻena noiʻi no ka pale ʻana i nā protein mai ka hoʻohaʻahaʻa ʻia e nā protease endogenous i ka wā o ka hoʻomaʻemaʻe ʻana a me ka nānā ʻana i ka protein. Ma ka pale ʻana i ka hana protease, kōkua ʻo leupeptin i ka mālama ʻana i ka pono a me ke kūpaʻa o nā protein i manaʻo ʻia, e ʻae ana i nā mea noiʻi e loaʻa nā hopena pololei i kā lākou mau hoʻokolohua. I loko o ke olaola cell, hoʻokomo pinepine ʻia ʻo leupeptin i loko o nā buffers lysis cell e pale aku i ka hoʻohaʻahaʻa ʻana o ka protein i ka wā o ka unuhi ʻana o nā protein cellular. Hōʻoia kēia e noho paʻa a hana nā protein i unuhi ʻia mai nā cell, e hiki ai i nā noi ma lalo e like me Western blotting, enzyme assays, a me nā haʻawina pilina protein-protein. Eia kekahi, hoʻohana pinepine ʻia ʻo leupeptin i nā haʻawina e noiʻi ana i ke kuleana o nā protease kikoʻī i nā kaʻina hana cellular. Ma ka pale koho ʻana i ka hana protease me leupeptin, hiki i nā mea noiʻi ke wehewehe i nā hana o nā protease i nā ala hōʻailona, ​​​​ka hoʻololi ʻana o ka protein, a me nā kaʻina hana physiological ʻē aʻe. Ma ke ʻano holoʻokoʻa, he mea hana pono ʻole ʻo leupeptin i ka noiʻi biochemical a me ke olaola cell, e hāʻawi ana i nā mea noiʻi i kahi ala hilinaʻi e kāohi i ka hana protease a pale i nā protein mai ka hoʻohaʻahaʻa ʻana. ʻO kona versatility, kūpaʻa, a me ka laulā ākea o ka inhibitory e hoʻolilo iā ia i mea waiwai nui no nā ʻano noi like ʻole e pili ana i ka hoʻomaopopo ʻana i nā ʻano hana paʻakikī e pili ana i nā kaʻina hana olaola.