MOPS CAS:1132-61-2 Kumukūʻai Mea Hana

ʻO ka hopena o ka MOPS (3-(N-morpholino) propanesulfonic acid) pili nui i kona hiki ke hoʻopaʻa a me ka hiki ke mālama i kahi pae pH paʻa.ʻO ka MOPS kahi pūhui zwitterionic, ʻo ia hoʻi, loaʻa iā ia kahi uku maikaʻi a maikaʻi ʻole, e hiki ai iā ia ke hana ma ke ʻano he pale pono i nā ʻōnaehana biological.

ʻO kekahi o nā noi nui o MOPS i loko o ka moʻomeheu cell, kahi i hoʻohana ʻia e mālama i ka pH o ka ulu ulu.Pono nā pūnaewele i ka pH paʻa no ka ulu a me ka hana maikaʻi loa, a kōkua ʻo MOPS i ka pale ʻana i ka waena a me ka pale ʻana i ka pH fluctuations i hiki ke hōʻino i ke olakino cell.

Hoʻohana pinepine ʻia ka MOPS i nā ʻenehana biology molekala e like me ka hoʻokaʻawale ʻana o DNA a me RNA, PCR (polymerase chain reaction), a me gel electrophoresis.Ma kēia mau noi, kōkua ʻo MOPS i ka hoʻopaʻa ʻana i ka pH o nā hui ʻana a me ka holo ʻana i nā buffers, e hōʻoiaʻiʻo ana i nā hopena pololei a hilinaʻi.

I ka hoʻopaʻa ʻana i ka protein, hiki ke hoʻohana ʻia ka MOPS ma ke ʻano he buffering agent i nā ʻenehana e like me ka hoʻomaʻemaʻe protein, ka helu protein, a me ka electrophoresis protein.Kōkua ia i ka mālama ʻana i ka pH kūpono e pono ai no ka paʻa ʻana o ka protein a me ka hana i kēia mau kaʻina hana.

Eia hou, hiki ke hoʻohana ʻia ka MOPS i nā hopena enzyme a me nā haʻawina kinetics enzyme.Hiki i kona hiki ke hoʻopaʻa i ka mālama ʻana i nā kūlana pH maikaʻi loa, he mea koʻikoʻi ia no ka hana enzyme a me nā ana kinetic pololei.