MOPS CAS:1132-61-2 Kumukūʻai Mea Hana

ʻO ka hopena o ka MOPS (3-(N-morpholino)propanesulfonic acid) e pili nui ana i kona hiki ke hoʻopaʻa a me ka hiki ke mālama i kahi pae pH paʻa. He hui zwitterionic ka MOPS, ʻo ia hoʻi, aia i loko o ia ka uku maikaʻi a me ka uku maikaʻi ʻole, e hiki ai iā ia ke hana ma ke ʻano he buffer maikaʻi i nā ʻōnaehana olaola.

ʻO kekahi o nā hoʻohana koʻikoʻi o MOPS ʻo ia ka moʻomeheu cell, kahi e hoʻohana ʻia ai e mālama i ka pH o ka medium ulu. Pono nā cell i kahi pH paʻa no ka ulu maikaʻi loa a me ka hana, a kōkua ʻo MOPS i ka buffering i ka medium a me ka pale ʻana i nā loli pH e hiki ke hōʻino i ke olakino cell.

Hoʻohana pinepine ʻia ʻo MOPS i nā ʻano hana biology molekala e like me ka hoʻokaʻawale ʻana o ka DNA a me ka RNA, PCR (polymerase chain reaction), a me ka gel electrophoresis. Ma kēia mau noi, kōkua ʻo MOPS i ka hoʻopaʻa ʻana i ka pH o nā hui hopena a me ka holo ʻana o nā buffers, e hōʻoiaʻiʻo ana i nā hopena pololei a hilinaʻi hoʻi.

I ka loiloi protein, hiki ke hoʻohana ʻia ʻo MOPS ma ke ʻano he mea hoʻopaʻa i nā ʻano hana e like me ka hoʻomaʻemaʻe protein, ka helu ʻana o ka protein, a me ka electrophoresis protein. Kōkua ia i ka mālama ʻana i ke kaiapuni pH kūpono e pono ai no ke kūpaʻa a me ka hana o ka protein i ka wā o kēia mau kaʻina hana.

Eia kekahi, hiki ke hoʻohana ʻia ʻo MOPS i nā hopena enzyme a me nā haʻawina kinetics enzyme. ʻO kona hiki ke hoʻopaʻa i ka buffering e ʻae i ka mālama ʻana i nā kūlana pH kūpono, he mea nui ia no ka hana enzyme a me nā ana kinetic pololei.