NBD chloride CAS: 10199-89-0

Ka lepili ʻana o Covalent: Hiki i ka NBD chloride ke hana i nā pilina covalent me nā hui nucleophilic i loko o nā biomolecules, e like me nā amines mua, thiols, a me nā hui hydroxyl. ʻAe kēia no ka lepili kikoʻī o kēia mau biomolecules no ka ʻike ʻana a me nā kumu kiʻi.

Hoʻonui ʻana i ka fluorescence: Ke hana ka NBD chloride me nā nucleophiles, hana ia i kahi huahana fluorescent i hōʻailona ʻia me ka NBD. Hōʻike kēia huahana i hōʻailona ʻia i ka hoʻokuʻu fluorescence ikaika, e lilo ia i mea pono no ka nānā ʻana a me ka hahai ʻana i nā molekala i hōʻailona ʻia.

ʻIke a me ke kikoʻī: He koʻikoʻi loa ka hoʻokuʻu ʻana o ka fluorescence o nā huahana i hōʻailona ʻia e NBD, e ʻae ana i ka ʻike ʻana i nā nui haʻahaʻa o nā molekala i hōʻailona ʻia. Eia kekahi, ʻo ka hoʻokumu ʻana o ka pilina covalent ma waena o NBD chloride a me nā nucleophiles e hāʻawi i ke kikoʻī, no ka mea, e hōʻoiaʻiʻo ana i kahi hoʻopili paʻa a kikoʻī o ka dye i ka biomolecule i manaʻo ʻia.

Ka lepili ʻana o ka biomolecule: Hiki ke hoʻohana ʻia ka NBD chloride e lepili i nā biomolecules like ʻole, me nā protein, peptides, nucleic acids, a me nā mole liʻiliʻi ʻē aʻe. Hiki i kēia i nā mea noiʻi ke noiʻi i ka localization, dynamics, a me nā pilina o kēia mau biomolecules i nā ʻōnaehana olaola.

ʻO ka microscopy fluorescence: Hiki ke ʻike ʻia nā biomolecules i hōʻailona ʻia me ka NBD me ka hoʻohana ʻana i ka microscopy fluorescence. ʻAe kēia ʻenehana i ka nānā ʻana i nā molekala i hōʻailona ʻia ma ka pae cellular a i ʻole subcellular, e hāʻawi ana i ka ʻike waiwai e pili ana i kā lākou hoʻolaha a me ke ʻano.

ʻO ke kahe ʻana o ka cytometry: Hiki ke kālailai ʻia a helu ʻia nā biomolecules i hōʻailona ʻia me ka NBD me ka hoʻohana ʻana i ka cytometry kahe. ʻAe kēia ʻenehana i ka nānā ʻana i nā cell a i ʻole nā ​​​​​​ʻāpana i hōʻailona ʻia, e hāʻawi ana i ka ʻike e pili ana i nā pae hōʻike a i ʻole nā ​​​​​​waiwai o ka ʻili o nā biomolecules i hōʻailona ʻia.

Ka lepili ʻana a me ka hahai ʻana i ka protein: Hiki ke hoʻohana ʻia ka NBD chloride e lepili i nā koena amino acid kikoʻī a i ʻole nā ​​​​hui hana i loko o nā protein. Hiki i kēia i nā mea noiʻi ke aʻo i ke ʻano o ka protein, ka pelu ʻana, ka dinamika, a me nā pilina i ka manawa maoli.