ʻO Nitro Blue Tetrazolium Chloride monohydrate CAS: 298-96-4

ʻO ka hopena Redox: Loaʻa iā NBT chloride monohydrate kahi hopena redox ke hoʻemi ʻia e kekahi mau enzymes a i ʻole nā ​​​​mea hoʻemi ʻē aʻe. ʻO kēia hopena ka hopena i ka hoʻokumu ʻia ʻana o kahi formazan precipitate polū ʻeleʻele, e hōʻike ana i ke alo a me ka hana o kēia mau enzymes reductase.

ʻIke ʻana i ka enzyme: Hoʻohana nui ʻia ka NBT chloride monohydrate i nā hoʻāʻo enzymatic e ʻike i ka hana o nā enzyme reductase like ʻole. No ka laʻana, hoʻohana ʻia no ka ʻike ʻana i nā dehydrogenases, succinate dehydrogenase, nitroreductases, a me nā enzyme oxidoreductase ʻē aʻe. ʻO ka precipitate formazan polū i hoʻokumu ʻia e hōʻike ana i ke alo a me ka hana o kēia mau enzymes, kahi e pono ai no ke ʻano enzyme a me nā haʻawina localization.

Hoʻoluʻu Histochemical: Hoʻohana ʻia ka NBT chloride monohydrate i nā ʻano hana hoʻoluʻu histochemical e ʻike i ka hoʻolaha ʻana a me ka hana o nā enzymes i loko o nā ʻiʻo. Hiki ke hoʻohana ʻia e aʻo i ka localization o nā enzymes i nā ʻano cell a i ʻole nā ​​​​ʻāpana ʻiʻo kikoʻī, e ʻae ana i nā mea noiʻi e hoʻomaopopo i ka hōʻike ʻana o ka enzyme a me ka hana i loko o nā laʻana olaola.

Nā hoʻāʻo ola o ke kelepona: Hoʻohana ʻia ʻo NBT chloride monohydrate i nā hoʻāʻo ola o ke kelepona e loiloi i ka hana metabolic a me ke kūlana redox o nā kelepona. ʻO ka hōʻemi ʻana o NBT chloride e hōʻike ana i ke alo o nā enzymes reductase ikaika i loko o nā kelepona, e hōʻike ana i ko lākou ola holoʻokoʻa a me ka hana metabolic.

Nā haʻawina microbiological: Hoʻohana ʻia ʻo NBT chloride monohydrate i nā haʻawina microbiological e ʻike i ke alo o nā enzymes microbial kikoʻī. Hiki ke hoʻohana ʻia kēia hui e loiloi i ka hana o kekahi mau enzymes i hana ʻia e ka bacteria a i ʻole fungi, e kōkua ana i ka ʻike ʻana a me ke ʻano o nā ʻano microbial.