NSP-SA-NHS CAS:199293-83-9 Kumukūʻai Mea Hana

ʻO NSP-SA-NHS, i ʻike ʻia hoʻi ʻo N-succinimidyl S-acetylthioacetate N-hydroxysuccinimide ester, he hui i hoʻohana pinepine ʻia ma ke ʻano he reagent crosslinking thiol-specific i nā hopena bioconjugation. ʻO kona hopena nui ka hoʻokumu ʻana o nā pilina thioester paʻa ma waena o nā hui thiol i loaʻa ma nā biomolecules, e like me nā protein a i ʻole nā ​​peptides.

ʻO ka hoʻohana ʻana o NSP-SA-NHS ma ke kahua o ka hoʻololi ʻana i ka protein a me ka hoʻopaʻa ʻana. ʻO kekahi o kāna mau hoʻohana koʻikoʻi:

Ka lepili ʻana i ka protein: Hoʻohana ʻia ʻo NSP-SA-NHS e hoʻopili covalently i nā lepili, e like me nā dyes fluorescent a i ʻole biotin, i nā protein a i ʻole peptides. ʻAe kēia i ka ʻike ʻana, ka ʻike ʻana, a me ka hahai ʻana i nā biomolecules i lepili ʻia i nā hoʻāʻo olaola like ʻole.

Nā pilina protein-protein: Hiki ke hoʻohana ʻia ʻo NSP-SA-NHS e hoʻopili i nā protein e launa pū ana e aʻo i nā pilina protein-protein. Hoʻohana pinepine ʻia kēia i nā ʻano hana e like me ka co-immunoprecipitation a i ʻole nā ​​​​​​hoʻāʻo pull-down e ʻike i nā hoa hoʻopaʻa a i ʻole e aʻo i nā paʻakikī protein.

Ka hoʻopaʻa ʻana i ka protein: ʻAe ʻo NSP-SA-NHS i ka hoʻopili covalent o nā protein a i ʻole peptides ma luna o nā ʻili paʻa, me nā momi agarose, nā momi magnetic, a i ʻole nā ​​​​microplates. He mea pono kēia i nā noi e like me ka hoʻomaʻemaʻe affinity, ka nānā ʻana i nā lāʻau lapaʻau, a i ʻole ka hoʻomohala ʻana o biosensor.

Hoʻololi ʻili: Hiki ke hoʻohana ʻia ʻo NSP-SA-NHS e hoʻololi i nā ʻili, e like me nā paheʻe aniani a i ʻole nā ​​nanoparticles, me nā protein a i ʻole peptides, e hana ana i nā ʻili i hana ʻia e biomolecule no nā noi like ʻole e like me nā diagnostics, nā ʻōnaehana lawe lāʻau lapaʻau, a i ʻole nā ​​​​kahua bio-sensing.