Phenylgalactoside CAS:2818-58-8

Ka hopena i ka hana enzyme: Hoʻohana mau ʻia ka Phenylgalactoside e ana i ka hana o ka enzyme β-galactosidase.Ke hoʻokahe ʻia ka phenylgalactoside e β-galactosidase, hoʻokuʻu ia i ka p-nitrophenol.Hiki ke ana ʻia ka hōʻiliʻili ʻana o ka p-nitrophenol, e hāʻawi ana i nā ʻike i ka hana o β-galactosidase.Hoʻohana ʻia kēia hopena i nā noi e like me ka hoʻāʻo ʻana o ka enzyme a me nā ʻōnaehana screening.

Ke kālailai hōʻike Gene: Hoʻohana pinepine ʻia ʻo Phenylgalactoside ma ke ʻano he substrate i nā hoʻokolohua biology molecular e aʻo ai i ka hōʻike gene.Hoʻohui pinepine ʻia ka gene lacZ, kahi e hoʻopili ai i ka β-galactosidase me nā kaʻina hoʻoponopono o nā ʻano genes ʻē aʻe.ʻO ka hōʻike ʻana o ka gene lacZ a me ka hydrolysis o phenylgalactoside e β-galactosidase hiki ke hōʻike i ke ʻano hōʻike a me ke kiʻekiʻe o ka gene i aʻo ʻia.

Nā ʻōnaehana nānā: ʻO Phenylgalactoside kahi mea nui o nā ʻōnaehana nānā e hoʻohana i ka hana β-galactosidase.ʻO kekahi laʻana i ʻike nui ʻia ʻo ke ʻano kānana polū-keʻokeʻo, i hoʻohana ʻia no ka ʻike ʻana i nā cell recombinant a i hoʻololi ʻia i nā hoʻokolohua biology molecular.ʻO nā kolone i lawe maikaʻi i ka DNA recombinant a i ʻole ka hoʻohui hou ʻana i ka genetic e hōʻike i ka β-galactosidase, e alakaʻi ana i ka hydrolysis o phenylgalactoside a me ka hoʻokumu ʻana i kahi kala uliuli.

Hoʻomaʻemaʻe protein: I kekahi mau hihia, hiki ke hoʻohana ʻia ka phenylgalactoside ma ke ʻano he ligand no ka chromatography affinity e hoʻomaʻemaʻe i nā protein i hoʻopaʻa pono ʻia a hoʻāla ʻia e β-galactosidase.Loaʻa paha i ka pūmua o ka hoihoi kahi hōʻailona pili a i ʻole kahi hōʻailona fusion i loaʻa kahi kikowaena β-galactosidase-binding domain.Ma ke kau ʻana i ka hui pūmua ma o ke kolamu me ka phenylgalactoside immobilized, hiki ke koho ʻia ka protein i makemake ʻia a hoʻopau ʻia.