piperazine-1,4-bis(2-ethanesulfonic acid) disodium paʻakai CAS:76836-02-7

Nā hopena:

Nā waiwai hoʻopaʻa: Hiki ke hoʻohana ʻia nā PIPES no ka mālama ʻana i nā pae pH mau i loko o kahi kikoʻī kikoʻī, no ka mea he maikaʻi ia i ka pale ʻana i ka pae pH physiological o 6.1-7.5.Hoʻohana kēia i nā hoʻokolohua olaola like ʻole kahi koʻikoʻi ka mana pH.

Paʻa: Paʻa ʻo PIPES ma luna o kahi ākea o nā wela, kūpono ia no ka hoʻohana ʻana i nā hoʻokolohua i hana ʻia ma lalo o nā kūlana like ʻole.

Nā noi:

Moʻomeheu pūnaewele: Hiki ke hoʻohana ʻia nā PIPES ma ke ʻano he pale i nā ʻenehana moʻomeheu cell, e like me ka mālama ʻana i ka pH o ka media a i ʻole nā ​​mea pale i hoʻohana ʻia no ka ulu ʻana a me ka mālama ʻana.

Nā haʻawina protein a me ka enzyme: Hoʻohana mau ʻia ka PIPES i nā noiʻi protein a me nā enzyme e mālama i ka pH paʻa i ka wā o nā hopena like ʻole, ʻoi aku ka nui o nā mea e pili ana i nā enzyme koʻikoʻi a i ʻole nā ​​​​protein e pili ana i nā loli pH.

Electrophoresis: Hiki ke hoʻohana ʻia nā PIPES ma ke ʻano he pale i nā noi electrophoresis gel, e kōkua ana i ka mālama ʻana i nā kūlana pH maikaʻi loa no ka DNA a i ʻole ka hoʻokaʻawale protein.

Nā ʻenehana biology molecular: Hiki ke hoʻohana ʻia nā PIPES ma ke ʻano he pale i nā ʻenehana biology molecular like ʻole, e komo pū ana me DNA/RNA extraction, PCR, a me DNA sequencing, e hōʻoia ana i nā hopena pololei ma ka mālama ʻana i nā kūlana pH paʻa.