PMSF CAS:329-98-6 Kumukūʻai Mea Hana

Hopena:

He mea hoʻopale serine protease ka PMSF e hoʻopaʻa mau ana i ka pūnaewele hana o nā serine proteases, a laila e ālai ana i kā lākou hana.

Hoʻololi ia i nā koena serine ma o nā pilina covalent, e alakaʻi ana i ka inactivation enzyme.

Hiki i ka PMSF ke pale i nā protein mai ka hōʻino ʻia ma o ke kāohi ʻana i ka hana protease, ʻoiai nā serine proteases.

Nā noi:

Noiʻi Biochemical: Hoʻohana pinepine ʻia ka PMSF i ka wā o ka hoʻomaʻemaʻe protein a me nā kaʻina hana hoʻokaʻawale e pale aku i ka hōʻino ʻana o ka protein. Kōkua ia i ka mālama ʻana i ka pono a me ke kūpaʻa o ka protein i manaʻo ʻia.

Ka pale ʻana i ka enzyme: ʻO ka PMSF kahi mea hana koʻikoʻi no ka hoʻopau ʻana i nā serine proteases i ka wā o nā hoʻāʻo enzyme a i ʻole nā ​​​​haʻawina biochemical. Ma ka pale ʻana i ka hana o kēia mau proteases, hiki i nā mea noiʻi ke noiʻi i ke kuleana o nā serine proteases i nā kaʻina hana olaola like ʻole.

Nā haʻawina proteolysis: Hiki ke hoʻohana ʻia ka PMSF e kāohi a hoʻoponopono paha i ka hana protease i loko o nā lysates cell a i ʻole nā ​​​​​​mea i unuhi ʻia. He mea maikaʻi kēia no ke aʻo ʻana i nā hanana proteolytic e pili ana i nā kaʻina hana cellular a i ʻole ke pono e hoʻoponopono ʻia ka proteolysis i ka hoʻonohonoho hoʻokolohua.

Ka pale ʻana o ka protease maʻamau: ʻOiai ke kuhikuhi pono nei ka PMSF i nā serine proteases, hiki iā ia ke loaʻa kekahi hopena pale i kekahi mau cysteine ​​proteases. Hoʻohana ʻia ia ma ke ʻano he mea pale protease maʻamau i ka wā e hana ana me nā laʻana protein e hōʻemi i ka hoʻohaʻahaʻa proteolytic.