Proteinase K CAS:39450-01-6 Kumukūʻai Mea Hana

ʻO ka hoʻoheheʻe ʻana a me ka hoʻomaʻemaʻe ʻana i ka protein: Hoʻohana pinepine ʻia ʻo Proteinase K i nā kaʻina hana hoʻomaʻemaʻe protein e wehe i nā protein a i ʻole nā ​​​​mea haumia i makemake ʻole ʻia. Hiki iā ia ke ʻoki pono i nā pilina peptide a hoʻoheheʻe i nā protein, e kōkua ana i ka hoʻokaʻawale ʻana a me ka hoʻomaʻemaʻe ʻana o nā protein i manaʻo ʻia.

Ka Hoʻokaʻawale ʻana i ka Waikawa Nucleic: Hoʻohana mau ʻia ʻo Proteinase K i nā kaʻina hana hoʻokaʻawale DNA a me RNA. Ma ka wāwahi ʻana i nā protein a me ka wehe ʻana i nā nucleases, kōkua ia i ka hoʻokaʻawale ʻana i ka DNA a i ʻole RNA kiʻekiʻe me ka ʻole o ka hōʻino ʻia. Hiki iā Proteinase K ke lawelawe i nā ʻano laʻana like ʻole, me ke koko, nā ʻiʻo, a i ʻole nā ​​​​​​pūnaewele.

Hoʻomaʻemaʻe PCR: He mea pono nō hoʻi ʻo Proteinase K i nā kaʻina hana hoʻomaʻemaʻe PCR. Hiki iā ia ke wehe i nā enzymes makemake ʻole ʻia, nā primers, nā nucleotides, a i ʻole nā ​​​​mea haumia ʻē aʻe e hiki ke hoʻopilikia i nā noi ma hope. Kōkua ka ʻai ʻana o Proteinase K i ka hoʻokō ʻana i nā huahana PCR maʻemaʻe i kūpono no ka nānā hou aku.

Ke Kuhi Manamana DNA: I ke kuhi manamana DNA a i ʻole ka loiloi forensic, hoʻohana ʻia ʻo Proteinase K e unuhi i ka DNA mai nā ʻano laʻana olaola like ʻole, e like me ke koko, ka lawai, a i ʻole ka lauoho. ʻO kona hiki ke hoʻohaʻahaʻa i nā protein e kōkua i ka wāwahi ʻana i nā membrane cell, e hoʻokuʻu ana i ka DNA no ka loiloi ma hope.

Histology a me Immunohistochemistry: Kōkua ʻo Proteinase K i ka loaʻa ʻana o ka antigen i nā ʻano hana histology a me immunohistochemistry. Hiki iā ia ke uhaki i nā cross-linkages i hana ʻia i ka wā o ka hoʻopaʻa ʻana i ka laʻana, e ʻae ana i ke komo maikaʻi ʻana i nā antigens a me ka hoʻomaikaʻi ʻana i ke ʻano o ka staining i nā ʻāpana kiko.

Hoʻopau ʻana i ka Enzyme: Hoʻohana pinepine ʻia ka Proteinase K e hoʻopau i nā enzymes. ʻO kona hiki ke hoʻohaʻahaʻa i nā enzymes e pale pono i ka haumia lawe ʻia mai nā hopena ma mua, e lilo ia i mea hana waiwai nui i nā hoʻokolohua biology molekala he nui.

Nā Haʻawina Hana a me ke ʻAno o ka Protein: Hoʻohana ʻia ʻo Proteinase K i ka noiʻi e aʻo i ke ʻano a me ka hana o ka protein. Ma ka ʻoki ʻana i nā pilina peptide ma nā wahi kikoʻī, hiki iā ia ke kōkua i ka ʻike ʻana i nā wahi koʻikoʻi a i ʻole nā ​​​​​​kikowaena hana i loko o nā protein.