ʻO ka Proteinase K no ka album tritirachium CAS: 39450-01-6

ʻO Proteinase K kahi enzyme i unuhi ʻia mai ka fungus Tritirachium album. He serine protease ākea ia, ʻo ia hoʻi hiki iā ia ke ʻoki i nā pilina peptide i loko o nā ʻano protein like ʻole. Ua ʻike ʻia ʻo Proteinase K no kona hiki ke hoʻohaʻahaʻa pono i nā protein, e lilo ia i mea hana waiwai nui i nā noi like ʻole.

ʻO kekahi o nā hoʻohana nui o Proteinase K ʻo ia ka noiʻi biology molecular. Hoʻohana pinepine ʻia ia no nā protocols unuhi DNA a me RNA e wehe i nā protein e hiki ke hoʻopilikia i ka nānā ʻana ma hope. Kōkua ʻo Proteinase K i ka lysing cell a me ka hoʻohaʻahaʻa ʻana i nā protein cellular, e ʻae ana i ka hoʻokaʻawale ʻana o nā waikawa nucleic kiʻekiʻe.

Hoʻohana nui ʻia ʻo Proteinase K i ka nānā ʻana i ka protein a me nā haʻawina ʻano. Hiki ke hoʻohana ʻia no ka ʻeli ʻana o ka protein, e ʻae ana i ka hana ʻana o nā ʻāpana peptide i hiki ke kālailai ʻia e nā ʻenehana e like me ka spectrometry mass. Eia kekahi, he mea pono ʻo Proteinase K no ka hoʻonohonoho ʻana i ka protein a me ka ʻike ʻana i ke kūlana o nā waikawa amino i loko o kahi kaulahao polypeptide.

ʻO kekahi hoʻohana koʻikoʻi o Proteinase K ʻo ia ka hoʻomaʻemaʻe ʻana i nā ʻili. Hoʻohana pinepine ʻia ia i nā keʻena hoʻokolohua biology molekala e wehe i nā waikawa nucleic, nā protein, a me nā mea haumia ʻē aʻe mai nā lako hana hoʻokolohua a me nā ʻili. He paʻa loa ʻo Proteinase K a hana ma lalo o nā ʻano kūlana like ʻole, me nā mahana kiʻekiʻe a me nā pae pH like ʻole.