ʻO SYBR GREEN I Nucleic Acid Gel Stain CAS: 163795-75-3

ʻElectrophoresis gel DNA: Hoʻohui ʻia ka pena SYBR Green I i ka gel DNA ma hope o ka electrophoresis, a i ka wā e hōʻike ʻia ai i ka mālamalama UV, hoʻopaʻa ia i nā kāʻei DNA, e hana ana i ka fluorescence ʻālohilohi. Kōkua kēia ʻano pena i ka ʻike a me ke kālailai ʻana i nā ʻāpana DNA i hoʻokaʻawale ʻia e ka nui.

PCR Quantitative (qPCR): Hoʻohana pinepine ʻia ʻo SYBR Green I i ka qPCR e nānā i ka hoʻonui ʻana o nā moʻo DNA a i ʻole RNA i ka manawa maoli. Hoʻopaʻa ka dye i ka DNA kaula pālua i hana ʻia i ka wā o ka hoʻonui ʻana o PCR, a ua ana ʻia ka piʻi ʻana o ka ikaika fluorescence i kēlā me kēia pōʻaiapuni hoʻonui, e ʻae ana i ka helu ʻana o ka template nucleic acid hoʻomaka.

RNA gel electrophoresis: Hiki ke hoʻohana ʻia ʻo SYBR Green I no ka RNA gel electrophoresis, e ʻae ana i ka nānā ʻana a me ka nānā ʻana i nā ʻāpana RNA. He mea pono ia no ka loiloi kūpaʻa RNA a no ke aʻo ʻana i nā pae hōʻike RNA.

Ka lepili ʻana a me ka ʻike ʻana i ka waikawa nucleic: Hiki ke hoʻohana ʻia ʻo SYBR Green I e lepili i nā waikawa nucleic me ka fluorescence. Hiki ke hoʻokō ʻia kēia ma ka hoʻohui ʻana i ka dye i ka wā o ka synthesis waikawa nucleic a i ʻole ma ka hoʻohana ʻana i nā ʻano hana post-labeling. A laila hiki ke nānā ʻia a ʻike ʻia nā waikawa nucleic i lepili ʻia me ka hoʻohana ʻana i nā ʻōnaehana kiʻi fluorescent.