ʻAkika Trichloroacetica, paʻakai paʻakai CAS: 650-51-1

ʻO ka waikawa Trichloroacetic, ka paʻakai sodium, he kuleana koʻikoʻi ia i nā noi like ʻole i loko o nā kahua o ka biology molecular, biochemistry, a me ka kemika protein. Ma ke ʻano he reagent no ka hoʻoheheʻe ʻana o ka protein, hoʻohana nui ʻia ia i nā hoʻonohonoho hale hana no ka hoʻokaʻawale ʻana a me ka hoʻomaʻemaʻe ʻana i nā protein a me nā waikawa nucleic mai nā laʻana biological paʻakikī. I ka noiʻi biology molecular a me ka biochemistry, ʻo ka hoʻohana ʻana i ka waikawa trichloroacetic, ka paʻakai sodium, e hiki ai ke hoʻoheheʻe koho ʻia o nā protein mai nā lysates cell, nā supernatants moʻomeheu, a me nā wai ola ʻē aʻe. Hoʻomaʻalahi kēia kaʻina hana i ka wehe ʻana i nā mea haumia a me nā biomolecules i makemake ʻole ʻia, e ʻae ana i nā mea noiʻi e loaʻa nā laʻana protein i hoʻomaʻemaʻe ʻia no nā loiloi downstream e like me electrophoresis, Western blotting, mass spectrometry, a me nā assays enzyme. Eia kekahi, ʻo ka hiki o ka paʻakai ke hoʻoheheʻe i nā waikawa nucleic e lilo ia i mea hana koʻikoʻi no ka unuhi ʻana a me ka hoʻomaʻemaʻe ʻana o ka DNA a me RNA mai nā mea cellular. Ma ka hoʻokaʻawale koho ʻana i nā waikawa nucleic mai nā ʻāpana cellular ʻē aʻe, ʻo ka waikawa trichloroacetic, ka paʻakai sodium, e kōkua i ka hoʻokaʻawale ʻana i nā mea genetic kiʻekiʻe, he mea nui ia no ka cloning molecular, sequencing, PCR amplification, a me nā ʻano hana biology molecular ʻē aʻe. Ma waho aʻe o kona kuleana i ka hoʻomaʻemaʻe ʻana i ka protein a me ka nucleic acid, loaʻa ka trichloroacetic acid, ka paʻakai sodium, i ka noi ʻana i nā ala hōʻailona cellular, nā hoʻololi post-translational, a me nā pilina protein-protein. ʻO kona hoʻohana ʻana i ka hoʻomākaukau ʻana i ka laʻana a me ka fractionation protein he mea nui ia i ka wehewehe ʻana i nā kaʻina hana olaola paʻakikī a me ka hoʻomaopopo ʻana i nā hana o nā biomolecules pākahi i loko o nā ʻōnaehana ola. Ma keʻano holoʻokoʻa, ʻo ka trichloroacetic acid, ka paʻakai sodium, ke pāʻani nei i kahi kuleana koʻikoʻi i nā kahe hana hoʻokolohua o nā biologist molecular, biochemists, a me nā chemists protein, e hiki ai ke hoʻokaʻawale pono a me ka hoʻomaʻemaʻe ʻana i nā protein a me nā nucleic acid mai nā kumu olaola like ʻole. ʻO kona mau waiwai precipitation koho a me ka solubility wai e lilo ia i mea waiwai nui no ka hoʻolaha ʻana i ka noiʻi ʻepekema, ka ʻike lāʻau lapaʻau, a me ka hoʻomohala ʻana i nā noi biotechnological i manaʻo ʻia e wehewehe i nā intricacies o ka hana cellular a me ka hana ʻike genetic.