TRIS-Acetate CAS: 6850-28-8 Kumukūʻai Mea Hana

ʻO Tris-acetate (TRIS-Acetate) kahi mea hoʻohana maʻamau i nā hoʻokolohua biological a me biochemical.Aia ia me ka hui pū ʻana o tris (hydroxymethyl) aminomethane (Tris) a me ka waikawa acetic, e hana ana ma ke ʻano he pH regulator a stabilizer.ʻO ka pH o kahi TRIS-Acetate buffer maʻamau mai 7.4 a i 8.4.
ʻO ka hopena nui o TRIS-Acetate ka mālama ʻana i kahi pH paʻa, he mea koʻikoʻi ia no ka nui o nā hopena biological a me biochemical.Hana ia ma ke ʻano he pale ma ka hōʻemi ʻana i nā hoʻololi koʻikoʻi i ka pH e hiki mai ana ma muli o ka hoʻohui ʻana i nā waikawa a i ʻole nā ​​kumu i ka wā o nā kaʻina hoʻokolohua.
Loaʻa iā TRIS-Acetate nā noi like ʻole i ka molecular biology, biochemistry, a me biotechnology:
DNA a me RNA Electrophoresis: Hoʻohana maʻamau ʻia ʻo TRIS-Acetate ma ke ʻano he pale holo i ka agarose a me ka polyacrylamide gel electrophoresis.Hāʻawi ia i kahi kūlana pH paʻa i ka wā o ka hoʻokaʻawale ʻana o nā ʻāpana DNA a me RNA e pili ana i ko lākou nui.
ʻIkepili Protein: Hoʻohana ʻia nā mea pale TRIS-Acetate no ka electrophoresis protein, e like me SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis).Mālama ia i ka paʻa o ka protein a me ka hoʻokaʻawale ʻana i ka wā o ke kaʻina hana.
Nā Hana Enzyme: Hoʻohana pinepine ʻia ka TRIS-Acetate buffers i nā hoʻokolohua enzyme a me nā haʻawina.Hāʻawi ia i ka pae pH maikaʻi loa no nā hopena enzymatic like ʻole a kōkua i ka mālama ʻana i ka hana enzyme.
Cell and Tissue Culture: Hoʻohana ʻia ka TRIS-Acetate buffers i ka media moʻomeheu cell no ka mālama ʻana i ka pH kūpono no ka ulu a me ka hoʻonui.Kōkua ia i ka mālama ʻana i nā kūlana physiological e pono ai no ka ola o ke kelepona.