Tris(hydroxymethyl)nitromethane CAS:126-11-4

Ka hopena:

Kākoʻo Buffering: Ke hana nei ʻo Tris ma ke ʻano he mea hoʻolalelale maikaʻi ma muli o kona hiki ke ʻae a hāʻawi i nā protons, e mālama ana i kahi pae pH paʻa i nā hopena.Hoʻohana maʻamau ia ma ke ʻano he mea nui i nā ʻōnaehana buffer e hoʻopaʻa i ka pH o nā laʻana olaola a me nā hopena.

Nā noi:

Molecular Biology: Hoʻohana nui ʻia ʻo Tris ma ke ʻano he mea hoʻolalelale i nā ʻano hana biology molecular, me ka hoʻokaʻawale DNA a me RNA, PCR, gel electrophoresis, a me ka hoʻomaʻemaʻe protein.Kōkua ia i ka mālama ʻana i kahi kūlana pH paʻa, e ʻae i nā kūlana kūpono no nā hopena enzymatic a me nā pilina molekala.

Culture Cell: Hoʻohana pinepine ʻia ʻo Tris i ka media moʻomeheu cell e mālama i ka pH mau a me ke kaulike osmotic, e kākoʻo ana i ka ulu ʻana o ke kelepona olakino a me ka hiki.

ʻO ke Kemika Protein: Hoʻohana ʻia ʻo Tris i nā hoʻokolohua kemika protein, e like me ka solubilization protein, nā hōʻike kūpaʻa protein, a me nā haʻawina hoʻopaʻa ʻana i ka protein-ligand.Kōkua ia i ka mālama ʻana i ka pae pH i makemake ʻia, e hōʻoia i ka hoʻopili pono ʻana o ka protein a me ka hana.

Enzymology: Hoʻohana ʻia ʻo Tris i nā ʻano hoʻokolohua enzymatic e hoʻomaikaʻi i nā kūlana pH e pono ai no ka hana enzymatic.Hoʻopaʻa ia i nā hopena hilinaʻi a hiki ke hana hou ʻia, hiki ke ana pololei i nā kinetics enzyme a me nā haʻawina inhibition.

Nā Ho'āʻo Biochemical: Hoʻohana ʻia ʻo Tris ma ke ʻano he ʻāpana i ka nui o nā hoʻokolohua biochemical ma muli o kāna mau mea pale.Mālama ʻo ia i ka pH mau i ka wā colorimetric, spectrophotometric, a me ka enzymatic assays, hoʻonui i ka pololei a me ka hilinaʻi o nā hopena.