4-Methylumbelliferyl-beta-D-glucopyranoside CAS:18997-57-4

ʻO ka hopena o ka 4-Methylumbelliferyl-beta-D-glucopyranoside (MUG) e lilo i mea pani no ka enzyme beta-glucosidase.Hoʻopau kēia enzyme i ka paʻa glucosidic o MUG, ka hopena i ka hoʻokuʻu ʻana o 4-Methylumbelliferone (4-MU).Hoʻohana pinepine ʻia ka MUG i ka ʻike ʻana i ka Escherichia coli (E. coli) i ka wai a me nā mea ʻai.Loaʻa iā E. coli ka enzyme beta-glucosidase, hiki iā ia ke hydrolyze MUG a hana i kahi hōʻailona fluorescent i mua o ke kukui ultraviolet (UV).Ke hoʻoheheʻe ʻia ka substrate MUG, hoʻopuka ka 4-MU i kahi fluorescence polū, e hoʻomaʻamaʻa i ka ʻike ʻana o nā huakini i loaʻa ka hana beta-glucosidase.Hoʻohana pinepine ʻia kēia ʻano hana i ka nānā ʻana i ke kaiapuni a me ka hoʻāʻo ʻana i ka palekana o ka meaʻai, no ka mea, hāʻawi ia i kahi ala wikiwiki a paʻakikī o ka ʻike ʻana i ka maʻi bacterial. ka noiʻi biochemistry a me ka molecular biology.Hiki i kāna fluorescence ke ana i nā kinetics enzyme a hiki ke hoʻohana ʻia e nānā i nā mea hoʻopaneʻe a i ʻole nā ​​​​mea hoʻoikaika o ka hana beta-glucosidase. Ma ke ʻano holoʻokoʻa, ʻo MUG kahi pūhui versatile e loaʻa ai ka hoʻohana ākea ma nā kahua o ka microbiology, biochemistry, a me ka biology molecular no ka ʻike. ʻO ka hana beta-glucosidase a me ka ʻike ʻana i nā hua bacteria e hana i kēia enzyme.