4-Nitrophenyl Beta-D-glucuronide CAS:10344-94-2

ʻIke i ka hana β-glucuronidase: Hoʻohana mau ʻia ʻo 4-NPBG ma ke ʻano he substrate chromogenic e loiloi i ka hele ʻana a me ka hana o ka β-glucuronidase i nā laʻana olaola like ʻole.Hoʻopau ka enzyme i ka paʻa glycosidic o 4-NPBG, e hana ana i 4-nitrophenol, hiki ke ana ʻia me ka spectrophotometry.

Nā haʻawina lāʻau lapaʻau: No ka mea he kuleana koʻikoʻi ka β-glucuronidase i ka metabolism o nā lāʻau lapaʻau a me nā xenobiotics, hiki ke hoʻohana ʻia ka 4-NPBG e loiloi i ka hana o kēia enzyme i nā haʻawina metabolism lāʻau.Kōkua ia i ka hoʻomaopopo ʻana i ka nui a me nā kinetics o nā hopena glucuronidation, he mea nui ia no ka hoʻomaʻemaʻe lāʻau a me ka bioavailability.

Nā haʻawina toxicology: Hiki ke hoʻopili ʻia a hoʻokuʻu ʻia kekahi mau pūhui toxicological ma ke ʻano o nā conjugates glucuronide.Ma ka hoʻohana ʻana i ka 4-NPBG ma ke ʻano he substrate, hiki i nā mea noiʻi ke nānā i ka hana o ka β-glucuronidase i loko o nā ʻiʻo like ʻole a i ʻole nā ​​laina kelepona e loiloi i nā mea ʻawaʻawa a i ʻole nā ​​hopena ʻino o nā pūhui.

Nā diagnostics Clinical: Hiki ke hoʻohana ʻia ke ana ʻana o ka hana β-glucuronidase me ka hoʻohana ʻana i ka 4-NPBG i nā hoʻonohonoho lāʻau lapaʻau e ʻike i kekahi mau maʻi a i ʻole nā ​​kūlana.Hiki ke hōʻike ʻia nā pae ʻokoʻa a i ʻole ka hana o ka β-glucuronidase i kekahi mau maʻi genetic, hana ʻole o ka ate, a i ʻole ka maʻi kanesa.