4-Nitrophenyl phosphate di(tris) paʻakai CAS:68189-42-4

Hoʻohana pinepine ʻia ka paʻakai 4-Nitrophenyl phosphate di(tris) ma ke ʻano he substrate no ka alkaline phosphatase i nā ʻano biochemical a me nā enzyme assays like ʻole. Ke hydrolyzed ʻia e ka alkaline phosphatase, hana ia i kahi huahana melemele hiki ke ana maʻalahi ʻia spectrophotometrically. Hoʻohana nui ʻia kēia hopena i nā noi e like me nā enzyme-linked immunosorbent assays (ELISAs) a me ka ʻike ʻana i ka hana phosphatase.

ʻO ka ikaika o ke kala melemele i hana ʻia ua kūlike pololei ia me ka hana enzyme, e ʻae ana i ka helu pololei ʻana o nā pae alkaline phosphatase i loko o nā laʻana olaola. ʻO kēia ka mea e pono ai i nā wahi lapaʻau a me nā noiʻi no ke aʻo ʻana i ka hana alkaline phosphatase i loko o nā cell, nā ʻiʻo, a me nā wai olaola.

Eia kekahi, paʻa ka paʻakai 4-Nitrophenyl phosphate di(tris) a hoʻoheheʻe nui ʻia i ka wai, e hoʻonui ana i kona kūpono no nā kūlana hoʻokolohua like ʻole. ʻO kona ʻike kiʻekiʻe a me ka maʻalahi o ke ana ʻana e lilo ia i koho kaulana ma ke ʻano he substrate i nā hoʻāʻo enzyme, e kōkua ana i ka loiloi ʻana o ka kinetics enzyme, ka hana enzyme, a me ka ʻike lāʻau.