5,5-Dithiobis (ʻakika 2-nitrobenzoic) CAS: 69-78-3

Ka helu ʻana o Thiol: Hoʻohana pinepine ʻia ka DTNB e loiloi i ka nui o nā thiols i loko o nā laʻana protein. He mea nui kēia ʻike no ke aʻo ʻana i nā pilina protein-protein, ka pelu ʻana o ka protein, a me nā hopena enzymatic e pili ana i nā hui thiol. ʻO ka pane colorimetric o DTNB e ʻae i ka helu ʻana o nā thiols ma o nā ana spectrophotometric.

Loiloi o ke Kūlana Redox: Ua hoʻohana ʻia ʻo DTNB e loiloi i ke kūlana redox i loko o nā pūnaewele, nā ʻiʻo, a i ʻole nā ​​​​​​laʻana olaola. Ma ke ana ʻana i nā loli i ka nui o ka thiol, hiki i nā mea noiʻi ke loaʻa ka ʻike i loko o ke kaumaha oxidative, ka hana antioxidant, a me nā ala hōʻailona redox.

Nā Hoʻāʻo Enzyme: ʻO ka hana kūikawā o DTNB me nā thiols e lilo ia i mea hana waiwai nui no nā hoʻāʻo enzyme. Nui nā enzymes, e like me glutathione-S-transferases, cystathionine-γ-lyase, a me thioredoxin reductase, e hilinaʻi ana i ka thiol a hiki ke helu ʻia a hoʻāʻo ʻia paha me ka hoʻohana ʻana iā DTNB.

Ka Loiloi Paʻa o nā Pūhui Metal-Sulfur: Hoʻohana ʻia ʻo DTNB e loiloi i ke kūpaʻa a me ka hana hou ʻana o nā hui metal-sulfur a me nā metalloproteins. Hiki i ka hui ke ʻike a helu i ke alo o nā thiols i komo i ka chelation o nā ion metala, e hāʻawi ana i ka ʻike koʻikoʻi e pili ana i nā pilina metal-sulfur a me ke kemika hoʻonohonoho.