5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium paʻakai CAS:18656-96-7

ʻO ka hopena o ka 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt (X-glucuronide) ʻo ia kona hiki ke hana i kahi precipitate polū a i ʻole ka loli kala i ke alo o ka enzyme beta-D-glucuronidase. Hana ʻia kēia hopena enzymatic i ka wā e ʻoki ʻia ai ka pilina glycosidic ma waena o ka hui indole a me ka ʻāpana waikawa glucuronic e ka beta-D-glucuronidase.

ʻO ka hoʻohana ʻana o X-glucuronide ka mea nui i ka biology molekala a me nā hoʻokolohua enzymology. Hoʻohana pinepine ʻia ia ma ke ʻano he substrate e ʻike ai i ka hana o ka beta-D-glucuronidase. ʻO kekahi mau noi kikoʻī:

Nā hoʻāʻo gene hōʻike: Hoʻohana pinepine ʻia ʻo X-glucuronide ma ke ʻano he substrate i nā hoʻāʻo gene hōʻike kahi e hoʻohana ʻia ai ka beta-D-glucuronidase ma ke ʻano he enzyme hōʻike. Ma ka hoʻohui ʻana i ka ʻāpana hoʻolaha a i ʻole ke kaʻina coding o kahi gene hoihoi i ka gene beta-D-glucuronidase, hiki i nā mea noiʻi ke ana i ka hōʻike a i ʻole ka hana o ka gene ma ka nānā ʻana i ka hoʻokumu ʻia ʻana o kahi precipitate polū a i ʻole ka loli kala i ka wā e hoʻohui ʻia ai ʻo X-glucuronide.

Nā hoʻāʻo hoʻololi bacteria a me ka hū: Hiki ke hoʻohana ʻia ʻo X-glucuronide e ʻike i ka hoʻololi kūleʻa o ka bacteria a i ʻole ka hū me kahi vector e loaʻa ana ka gene beta-D-glucuronidase. Hiki i nā cell i hoʻololi ʻia e hōʻike ana i ka enzyme ke hydrolyze i ka X-glucuronide, e hana ana i kahi loli kala i ʻike ʻia a i ʻole ka precipitate.

Hoʻoluʻu histochemical: Hoʻohana ʻia ʻo X-glucuronide i nā ʻano hana hoʻoluʻu histochemical e ʻike i ke alo a me ka hoʻolaha ʻana o ka hana beta-D-glucuronidase i loko o nā ʻiʻo a i ʻole nā ​​​​​​pūnaewele. ʻAe kēia hoʻoluʻu i ka localization a me ka ʻike ʻana o nā pūnaewele e hōʻike ana i ka enzyme.