ʻAkika 8-Anilino-1-naphthalenesulfonic CAS:82-76-8

ʻŌmole fluorescent: Hoʻohana nui ʻia ʻo ANS ma ke ʻano he ʻōmole fluorescent e aʻo ai i nā loli conformational protein, nā kaʻina hana pelu/wehe ʻana o ka protein, a me nā pilina protein-ligand. Hoʻopaʻa ia i nā ʻāpana hydrophobic a i ʻole nā ​​​​wahi hydrophobic i hōʻike ʻia i loko o nā protein, e hopena ana i kahi hoʻokuʻu fluorescence hiki ke nānā ʻia me ka hoʻohana ʻana i ka spectroscopy fluorescence.

Ka ʻike ʻana i ka hydrophobicity: He ʻike koʻikoʻi ʻo ANS i nā loli i ka hydrophobicity o ka microenvironment. Hiki ke hoʻohana ʻia e ʻike a helu i nā surfactants, nā detergents, a me nā mea hydrophobic ʻē aʻe.

Loiloi kūpaʻa protein: Hoʻohana pinepine ʻia ʻo ANS i nā hoʻāʻo kūpaʻa wela e loiloi i ke kūpaʻa o nā protein. Ma ka nānā ʻana i ka ikaika fluorescence o nā protein i hoʻopaʻa ʻia e ANS ma lalo o nā mahana like ʻole, hiki ke hoʻoholo i nā ʻano denaturation thermal a me ka mahana hoʻoheheʻe (Tm) o ka protein.

Nā haʻawina hōʻuluʻulu protein: Hiki ke hoʻohana ʻia ʻo ANS e aʻo i nā kaʻina hana hōʻuluʻulu protein, e like me ke kūkulu ʻia ʻana o nā fibrils amyloid. He ʻike ka dye i nā ʻāpana hydrophobic i hōʻike ʻia i loko o nā hōʻuluʻulu protein, e lilo ia i mea hana waiwai no ka nānā ʻana i ka holomua a me ke kinetics o ka hōʻuluʻulu ʻana.

ʻAno o nā pilina protein-protein: Ua hoʻohana ʻia ʻo ANS e noiʻi i nā pilina protein-protein. Ke launa pū nā protein, aia paha kahi loli i ko lākou hydrophobicity, hiki ke nānā ʻia me ka hoʻohana ʻana i ka fluorescence ANS.

Ka ʻike ʻana a me ka hoʻomohala ʻana i nā lāʻau lapaʻau: Hiki ke hoʻohana ʻia ʻo ANS i ka nānā ʻana i nā moho lāʻau lapaʻau kūpono. Hiki iā ia ke kōkua i ka loiloi ʻana i ka pilina paʻa a me ka launa pū ʻana o nā mole liʻiliʻi me nā protein i manaʻo ʻia, e ʻae ana i nā mea noiʻi e ʻike i nā hui e hiki ke hoʻololi i ka hana protein.