N-Ethylmaleimide CAS:128-53-0 Kumukūʻai Mea Hana

Hoʻololi ʻana i ka Protein: Hoʻololi mau ʻo NEM i nā koena cysteine ​​i loko o nā protein ma o ka hoʻokumu ʻana i kahi adduct covalent. Hiki i kēia hoʻololi ke hoʻopilikia i ka hana protein, ka hoʻonohonoho ʻana, a me nā pilina. ʻO ka hana a NEM me nā hui sulfhydryl e lilo ia i mea hana waiwai no ke aʻo ʻana i ka hana protein a me nā kaʻina hana e pili ana i ka thiol.

Ka Pale ʻana i nā Enzymes: Hilinaʻi ka nui o nā enzymes i nā hui thiol no kā lākou hana catalytic. Ma ka pane ʻana me nā hui thiol, hiki iā NEM ke kāohi koho i nā enzymes kikoʻī. Ua hoʻohana nui ʻia kēia e aʻo i nā kinetics enzyme, e hoʻomaopopo i nā ʻano hana enzymatic, a nānā i nā mea hiki ke pale i ka enzyme.

Nā Haʻawina Pilina Protein-Protein: Hoʻohana pinepine ʻia ʻo NEM e noiʻi i nā pilina protein-protein. Ma ke kāohi koho ʻana i nā koena cysteine ​​​​reactive, pale ʻo NEM i ka hoʻokumu ʻia ʻana o nā pilina disulfide ma waena o nā protein e launa pū ana, a laila e hoʻopilikia ana i kā lākou pilina. ʻAe kēia i nā mea noiʻi e aʻo i ke kuleana o nā cysteines kikoʻī i nā pilina protein-protein.

Nā Haʻawina Hoʻoponopono Redox: Hoʻohana ʻia ʻo NEM i ke aʻo ʻana i nā ʻano hana hoʻoponopono redox i loko o nā protein. Ma ka hoʻololi ʻana i nā cysteines reactive, hiki iā NEM ke hoʻopaʻa i nā ʻano protein kikoʻī a pale i ka oxidation makemake ʻole ʻia a i ʻole ka hōʻemi ʻana o nā koena cysteine. Kōkua kēia i ka hoʻomaopopo ʻana i ke kuleana o ka hōʻailona redox a me nā ala hoʻoponopono redox-sensitive.

Nā Haʻawina Hoʻopili: Hiki ke hoʻohana ʻia ʻo NEM ma ke ʻano he mea hoʻopili e hoʻopaʻa i nā hui protein a i ʻole nā ​​pilina protein-protein. Ma ka hoʻololi ʻana i nā koena cysteine, hiki iā NEM ke hana i nā pilina covalent ma waena o nā protein e launa pū ana, e ʻae ana i nā mea noiʻi e noiʻi i nā hui protein a me ko lākou mau ʻano. He mea pono loa kēia ʻano hana i nā haʻawina biology structural a me proteomics.

Ka ʻIke ʻana i nā Lāʻau Lapaʻau: Ma muli o kona hiki ke launa pū me nā koena cysteine, ua hoʻohana ʻia ʻo NEM i ka noiʻi ʻike lāʻau lapaʻau. Hiki ke hoʻohana ʻia e ʻike a wehewehe i nā pahuhopu lāʻau lapaʻau kūpono a i ʻole e aʻo i ke ʻano kūikawā o nā lāʻau lapaʻau moho i nā protein waiwai i ka cysteine.