Nitrotetrazolium Blue Chloride CAS:298-83-9

ʻO ka Nitrotetrazolium Blue Chloride (NBT) kahi hōʻailona redox i hoʻohana mau ʻia i nā hoʻokolohua biological a me biochemical assays.He pauda melemele ʻeleʻele e lilo i polū ke hoʻemi ʻia, pono ia no ka ʻike ʻana i ka hele ʻana o kekahi mau enzymes a me ka hana metabolic.

ʻO ka hopena mua o ka NBT ka hoʻokumu ʻana o kahi formazan polū i ka wā i hoʻemi ʻia e kekahi mau enzymes.Hiki i kēia hoʻololi kala ke ʻike maka a spectrophotometric paha o ka hana enzyme.

Loaʻa i ka NBT nā noi like ʻole i ka noiʻi a me nā diagnostics.Eia kekahi o kāna mau hoʻohana mua:

Nā ho'āʻo hana enzyme: Hiki ke hoʻohana ʻia ka NBT no ke ana ʻana i ka hana o nā dehydrogenases, i komo i loko o nā kaʻina hana e like me ka hanu cellular a me ka metabolism.Ma ka nānā ʻana i ka emi ʻana o ka NBT i formazan, hiki i nā mea noiʻi ke loiloi i ka hana o kēia mau enzymes.

Ka Loiloi o ka hana o ke kinikini immune: Hoʻohana mau ʻia ka NBT i ka hōʻike hōʻemi NBT no ka loiloi ʻana i ka hana hoʻohāhā o ka hanu o nā pūnaewele immune, ʻo ia hoʻi nā phagocytes.ʻO ka hoʻāʻo e ana i ka hiki o kēia mau cell ke hana i nā ʻano oxygen reactive, hiki ke hōʻemi i ka NBT a hoʻokumu i kahi polū polū.

Noiʻi Microbiology: Hoʻohana ʻia ʻo NBT i ka microbiology e aʻo i ka metabolism microbial a loiloi i ka hana o nā enzyme kikoʻī.No ka laʻana, ua hoʻohana ʻia e ʻike i nā reductases nitrate bacterial a i ʻole formazan-forming bacteria.

Nā haʻawina ola kino: ʻO ka hōʻemi ʻana o ka NBT e hiki i nā mea noiʻi ke loiloi i ka hana metabolic a me ka hiki o nā cell.Ma ka helu ʻana i ka ikaika o ka hua formazan polū, hiki ke hoʻoholo i ka helu o nā cell viable i loko o kahi hāʻina i hāʻawi ʻia.