Tricine CAS:5704-04-1 Kumukūʻai Mea Hana

I loko o ka biochemistry a me ka biology molecular, ʻo ka "tricine effect" e pili ana i ka hiki o ka tricine e hoʻomaikaʻi i ka hoʻokaʻawale ʻana a me ka hoʻonā ʻana o nā protein ma nā gels SDS-PAGE i hoʻohālikelike ʻia me nā ʻōnaehana kumu glycine.ʻO Tricine kahi amino acid liʻiliʻi ma mua o ka glycine a hiki ke komo maʻalahi i ka polyacrylamide gel matrix, e hopena i ka hoʻokaʻawale ʻana i ka protein.

ʻOi aku ka maikaʻi o ka ʻōnaehana tricine buffer no ka hoʻokaʻawale ʻana i nā protein paona haʻahaʻa haʻahaʻa (emi iho ma mua o 20 kDa) a me ka hoʻoholo ʻana i nā hui neʻe kokoke.Hoʻohana maʻamau ia i ka blotting Western, hoʻomaʻemaʻe protein, a me nā haʻawina hōʻike protein.Hoʻohana pū ʻia ʻo Tricine i hui pū me nā mea hoʻolalelale ʻē aʻe, e like me Bis-Tris a i ʻole MOPS, e hoʻonui i ka pae pH a hoʻomaikaʻi i ka hoʻonā protein i nā noi kikoʻī.

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