Tricine CAS:5704-04-1 Kumukūʻai Mea Hana

I loko o ka biochemistry a me ka biology molecular, ʻo ka "tricine effect" e pili ana i ka hiki o ka tricine ke hoʻomaikaʻi i ka hoʻokaʻawale ʻana a me ka hoʻonā ʻana o nā protein ma nā gels SDS-PAGE i hoʻohālikelike ʻia me nā ʻōnaehana glycine kuʻuna. He amino acid liʻiliʻi ʻo Tricine ma mua o ka glycine a hiki ke komo maʻalahi i loko o ka matrix gel polyacrylamide, e hopena ana i ka hoʻokaʻawale ʻana o ka protein.

He mea pono loa ka ʻōnaehana buffer tricine no ka hoʻokaʻawale ʻana i nā protein kaumaha molekala haʻahaʻa (ʻoi aku ka liʻiliʻi ma mua o 20 kDa) a me ka hoʻoponopono ʻana i nā hui neʻe kokoke. Hoʻohana pinepine ʻia ia i ka Western blotting, ka hoʻomaʻemaʻe protein, a me nā haʻawina hōʻike protein. Hoʻohana pū ʻia ʻo Tricine me nā mea buffering ʻē aʻe, e like me Bis-Tris a i ʻole MOPS, e hoʻonui i ka pae pH a hoʻomaikaʻi i ka hoʻonā protein i nā noi kikoʻī.

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