TRIS-Acetate CAS:6850-28-8 Kumukūʻai Mea Hana

ʻO Tris-acetate (TRIS-Acetate) kahi buffer i hoʻohana pinepine ʻia i nā hoʻokolohua olaola a me biochemical. Loaʻa iā ia ka hui pū ʻana o tris(hydroxymethyl)aminomethane (Tris) a me ka waikawa acetic, ka mea e hana ana ma ke ʻano he mea hoʻoponopono pH a me ka mea hoʻopaʻa. ʻO ka pH o kahi buffer TRIS-Acetate maʻamau mai 7.4 a 8.4.
ʻO ka hopena nui o TRIS-Acetate ka mālama ʻana i kahi pH paʻa, he mea nui ia no nā ʻano hana olaola a me nā biochemical he nui. Hana ia ma ke ʻano he buffer ma ka hoʻēmi ʻana i nā loli koʻikoʻi i ka pH e hiki mai ana ma muli o nā waikawa a i ʻole nā ​​​​​​base i hoʻohui ʻia i ka wā o nā kaʻina hana hoʻokolohua.
Loaʻa iā TRIS-Acetate nā noi like ʻole i ka biology molecular, biochemistry, a me biotechnology:
ʻO ka DNA a me ka RNA Electrophoresis: Hoʻohana pinepine ʻia ʻo TRIS-Acetate ma ke ʻano he buffer holo i ka agarose a me ka polyacrylamide gel electrophoresis. Hāʻawi ia i kahi kūlana pH paʻa i ka wā e hoʻokaʻawale ai i nā ʻāpana DNA a me RNA ma muli o ko lākou nui.
Ka Nānā ʻana i ka Protein: Hoʻohana ʻia nā buffers TRIS-Acetate no ka electrophoresis protein, e like me SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis). Hōʻoiaʻiʻo ia i ke kūpaʻa o ka protein a me ka hoʻokaʻawale ʻana i ka wā o ke kaʻina hana.
Nā Hana Enzyme: Hoʻohana pinepine ʻia nā buffers TRIS-Acetate i nā hoʻāʻo enzyme a me nā haʻawina. Hāʻawi ia i ka pae pH kūpono no nā ʻano hana enzymatic like ʻole a kōkua i ka mālama ʻana i ka hana enzyme.
Moʻomeheu Kelepona a me nā ʻIʻo: Hoʻohana ʻia nā buffers TRIS-Acetate i nā pāpaho moʻomeheu kelepona e mālama i ka pH kūpono no ka ulu ʻana a me ka hoʻonui ʻana o nā kelepona. Kōkua ia i ka mālama ʻana i nā kūlana physiological e pono ai no ke ola ʻana o nā kelepona.