3-HYDROXY-4-(5-NITROPYRIDYLAZO)PROPYLANI CAS:143205-66-7

ʻO ia kahi ʻimi fluorescent i loaʻa kahi hui nitrobenzoxadiazole (NBD), e hōʻike ana i nā waiwai fluorescence ikaika.Hāʻawi ka hui aldehyde i ka hōʻailona kikoʻī a me ka ʻike ʻana i nā biomolecules e like me nā protein, nucleic acids, a me nā lipids.

Hoʻohana pinepine ʻia ʻo NBD-aldehyde ma ke ʻano he dye reactive i nā assays no ka ʻike ʻana a me ka helu ʻana i ka protein a i ʻole peptide conjugation reactions, hoʻololi protein, a me nā pilina protein-protein.Hiki iā ia ke hoʻopaʻa inoa i nā pūʻulu amino manuahi ma nā protein a i ʻole peptides, e hana ana i nā paʻa covalent paʻa.He mea pono kēia no nā noi e like me immunocytochemistry, immunohistochemistry, a me ke komohana blotting.

Hiki ke hoʻohauʻoli ka fluorescence o NBD-aldehyde me ka ultraviolet a i ʻole ke kukui polū, a hoʻopuka ʻo ia i ka fluorescence ʻōmaʻomaʻo.Hāʻawi kēia waiwai i ka ʻike a me ka helu ʻana o nā biomolecules i hōʻailona ʻia me ka hoʻohana ʻana i ka microscopy fluorescence a i ʻole nā ​​ʻenehana spectroscopy.