4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4

Nā ʻāpana enzyme: Hiki ke hoʻohana ʻia ka 4NPM ma ke ʻano he pani no nā enzyme like ʻole, me nā glycosidases a me nā enzyme pili.Hoʻokaʻawale kēia mau enzymes i ka paʻa glycosidic ma waena o ka mannose a me 4NPM, e hopena i ka hoʻokuʻu ʻana o ka nitrophenyl moiety.Hiki ke ana ʻia ka nui o ka hydrolysis substrate ma ka nānā ʻana i ka hoʻopale ʻana o ka pūʻulu nitrophenyl i hoʻokuʻu ʻia ma kahi lōʻihi nalu.ʻAe kēia i nā mea noiʻi e loiloi i ka hana enzyme a me nā kinetics.

Nā ho'āʻo no ka metabolism carbohydrate: Ma ka hoʻohana ʻana i ka 4NPM ma ke ʻano he substrate, hiki i nā mea noiʻi ke aʻo i ka hana o nā enzyme i komo i ka metabolism carbohydrate, e like me alpha-mannosidases.ʻO kēia mau enzymes hydrolyze glycosidic paʻa i loko o nā pūhui mannose, a hiki ke ana ʻia kā lākou hana ma ka nānā ʻana i ka hoʻokuʻu ʻana o ka moiety nitrophenyl mai 4NPM.

Nā haʻawina Glycosylation: Hiki ke hoʻohana ʻia ʻo 4NPM i nā assays e noiʻi i nā enzyme i komo i nā kaʻina glycosylation.ʻO Glycosylation ke kaʻina hana o ka hoʻopili ʻana i nā molekala kō i nā protein a i ʻole nā ​​molekole ʻē aʻe, a nui nā enzyme i komo i kēia kaʻina hana.Ma ka hoʻohana ʻana i ka 4NPM ma ke ʻano he substrate ʻae, hiki i nā mea noiʻi ke ana i ka hoʻololi ʻana o kahi moiety kō i 4NPM e nā enzyme kikoʻī i komo i nā hopena glycosylation.

Ka nānā ʻana no nā mea hoʻopaneʻe enzyme a i ʻole nā ​​mea hoʻoulu: Hiki ke hoʻohana ʻia ka 4NPM i nā hoʻāʻo hōʻike kiʻekiʻe-throughput e ʻike ai i nā pūhui e kāohi a hoʻōla i nā enzyme kikoʻī.Ma ka loiloi ʻana i ka hopena o nā pūhui hoʻāʻo i ka hydrolysis a i ʻole ka hoʻololi ʻana o 4NPM e nā enzymes i hoʻopaʻa ʻia, hiki i nā mea noiʻi ke ʻike i nā mea lapaʻau kūpono a i ʻole nā ​​​​mea hoʻohana kemika pono no ke aʻo ʻana i ka hana enzyme.