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Heppso sodium CAS:89648-37-3 Kumukūʻai Mea Hana
ʻO ka paʻakai sodium N-[2-Hydroxyethyl]piperazine-N'-[2-hydroxypropanesulfonic acid] he hui kemika me ke ʻano C8H19N2NaO4S. He paʻakai sodium ia i loaʻa mai ka piperazine, nona nā hui hana hydroxyethyl a me hydroxypropanesulfonic acid. Hoʻohana pinepine ʻia ia i ka ʻoihana lāʻau lapaʻau ma ke ʻano he mea hoʻopaʻa a me ka mea hoʻopaʻa i nā ʻano lāʻau. Kōkua kēia hui i ka mālama ʻana i ka pH a me ke kūpaʻa o nā lāʻau lapaʻau.
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P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7
ʻO P-Nitrophenyl beta-D-lactopyranoside, i ʻike ʻia hoʻi ʻo PNPG, he hui i hoʻohana pinepine ʻia i nā hoʻāʻo enzymatic e ana i ka hana o ka beta-galactosidase, kahi enzyme e pili ana i ka metabolism carbohydrate. ʻO PNPG kahi substrate synthetic hiki ke ʻoki ʻia e beta-galactosidase, e hopena ana i ka hoʻokuʻu ʻia ʻana o kahi huahana melemele. Hiki ke helu ʻia ka nui o ka hydrolysis substrate spectrophotometrically ma ke ana ʻana i ka absorbance o ka huahana ma kahi nalu kikoʻī. ʻAe kēia i nā mea noiʻi e loiloi i ka hana a me nā kinetics o beta-galactosidase ma nā ʻano like ʻole, e like me ke aʻo ʻana i ka hana enzyme, ka nānā ʻana no nā mea hoʻopaʻa enzyme a i ʻole nā mea hoʻoulu, a i ʻole ka loiloi ʻana i nā hopena o nā mutations ma ka hana enzyme.
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popso sesquisodium CAS:108321-08-0
ʻO ka paʻakai sesquisodium Piperazine-N,N'-bis(2-hydroxypropanesulfonic acid) , i ʻike ʻia hoʻi ʻo PIPES sesquisodium salt, he hui kemika i hoʻohana ʻia ma ke ʻano he buffering agent i nā noi ʻepekema a me nā ʻoihana like ʻole. Kōkua ia i ka mālama ʻana i kahi pae pH paʻa i loko o nā hopena, ʻoi aku hoʻi i ka pae pH physiological. Hoʻohana pinepine ʻia ka paʻakai sesquisodium PIPES i nā media moʻomeheu cell, protein biochemistry, electrophoresis, nā ʻenehana biology molecular, a me nā ʻōnaehana lawe lāʻau. Hana ia ma ke ʻano he mea hoʻoponopono pH a me ka hoʻonui no ka hana enzyme a me ke kūpaʻa, e lilo ia i mea waiwai nui i nā ʻano noiʻi a me nā kaʻina hana ʻoihana.
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ACES CAS:7365-82-4 Kumukūʻai Mea Hana
ʻO ka N-(2-Acetamido)-2-aminoethanesulfonic acid, i ʻike ʻia hoʻi ʻo ACES, he mea hoʻopaʻa i hoʻohana pinepine ʻia i ka noiʻi ʻepekema. Kōkua ia i ka mālama ʻana i kahi pH paʻa i loko o nā hopena a he mea hoʻoheheʻe loa ia i ka wai. He haʻahaʻa ka ʻawahia o ACES a he liʻiliʻi ka hoʻopilikia ʻana me nā kaʻina hana biochemical, e kūpono ai no nā noi like ʻole. Hoʻohana pinepine ʻia ia ma ke ʻano he buffer i nā haʻawina hoʻopaʻa protein a me nā hoʻāʻo enzyme, kahi e mālama ai i nā kūlana kūpono no ka hoʻohālikelike protein a me ka hana enzyme.
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ʻO Phenylgalactoside CAS:2818-58-8
ʻO Phenylgalactoside, i ʻike ʻia hoʻi ʻo p-nitrophenyl β-D-galactopyranoside (pNPG), he substrate synthetic i hoʻohana pinepine ʻia i nā hoʻokolohua biochemical a me nā molecular biology. Hoʻohana pinepine ʻia ia e ʻike a ana i ka hana o ka enzyme β-galactosidase.
Ke hoʻoheheʻe ʻia ka phenylgalactoside e ka β-galactosidase, hoʻokuʻu ia i ka p-nitrophenol, he hui melemele ia. Hiki ke ana ʻia ka hoʻokuʻu ʻia ʻana o ka p-nitrophenol me ka hoʻohana ʻana i ka spectrophotometer, ʻoiai hiki ke ʻike ʻia ka absorbance o ka p-nitrophenol ma kahi nalu o 405 nm.
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DIPSO CAS:68399-80-4 Kumukūʻai Mea Hana
ʻO DIPSO ke kū nei no "Diisopropyl azodicarboxylate," kahi mea hoʻohana maʻamau i ka kemika organik. Hoʻohana nui ʻia ia i ka hopena Mitsunobu, ʻo ia ke ʻano no ka hoʻololi ʻana i nā waiʻona i nā hui hana like ʻole e like me nā esters, ethers, a i ʻole amines. Hana ʻo DIPSO ma ke ʻano he kumu o kahi waena reactive kiʻekiʻe i kapa ʻia ʻo azodicarboxylate, ka mea e hoʻomaʻamaʻa ai i kēia hoʻololi.
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4-Nitrophenyl-beta-D-glucopyranoside CAS:2492-87-7
ʻO 4-Nitrophenyl-beta-D-glucopyranoside kahi substrate i hoʻohana pinepine ʻia i nā hoʻokolohua biochemical e loiloi i ka hana o nā enzymes e like me β-glucuronidase. Hoʻoheheʻe ʻia kēia hui e ka enzyme, e hopena ana i ka hoʻokuʻu ʻia ʻana o 4-nitrophenol, hiki ke ana ʻia me ka hoʻohana ʻana i ka spectrophotometry. ʻO kona hoʻohana ʻana e hiki ai i nā mea noiʻi ke aʻo i nā ʻano like ʻole o ka metabolism lāʻau, toxicology, a me nā diagnostics lapaʻau e pili ana i nā hopena glucuronidation.
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Tris maleate CAS:72200-76-1
He hui kemika ʻo Tris maleate e lawelawe ana ma ke ʻano he buffer pH a me ka mea hoʻoponopono i nā ʻoihana like ʻole. Hoʻohana ʻia ia no ka mālama ʻana i nā pae pH paʻa a me ke kūʻē ʻana i nā loli i hoʻokumu ʻia e ka hoʻohui ʻana o nā waikawa a i ʻole nā kumu. Hoʻohana pinepine ʻia ʻo Tris maleate i ka noiʻi biochemical, ka hoʻomaʻemaʻe protein, nā kaʻina hana ʻoihana, a me ka kemika analytical. He mea kūpono loa ia i ka buffering ma nā pae pH haʻahaʻa a kaulana ʻia no kona versatility i ka mālama ʻana i nā kūlana pH kūpono.
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MOBS CAS:115724-21-5 Kumukūʻai Mea Hana
ʻO ka 4-morpholin-4-ylbutane-1-sulfonic acid, i ʻike ʻia hoʻi ʻo MOBʻO S, he hui organik synthetic i hoʻohana ʻia ma ke ʻano he buffering agent i ka noiʻi biological a me biochemical. He paʻa a hiki ke mālama i kahi pH paʻa ma ka pae neutral a i ʻole alkaline iki. MOBHoʻohana nui ʻia ʻo S i ka moʻomeheu cell, nā hoʻāʻo enzyme, nā ʻano hana biology molecular, a me ka electrophoresis. He kūpono ia me nā ʻano reagents like ʻole a ua ʻike ʻia no kona kūpaʻa a me ke kūʻē ʻana i nā loli o ka mahana a me ka pH.
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1,2,3,4,6-penta-O-acetyl-alpha-D-galactopyranose CAS:4163-59-1
ʻO 1,2,3,4,6-penta-O-acetyl-alpha-D-galactopyranose kahi hui kemika no ka ʻohana o nā kalapona. He hua ia o ka alpha-D-galactopyranose, kahi kō kūlohelohe. Loaʻa i kēia hui kikoʻī ʻelima mau hui acetyl i hoʻopili ʻia i nā hui hydroxyl kikoʻī ma ka molekole kō. Hoʻohana pinepine ʻia ia i nā noi kemika a me nā lāʻau lapaʻau like ʻole, me ke ʻano he mea hoʻomaka no ka synthesis o nā hui ʻē aʻe. Hoʻonui kona ʻano acetylated i kona kūpaʻa a me ka reactivity, e lilo ia i poloka hale pono i ka kemika organik.
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POPSO CAS:68189-43-5 Kumukūʻai Mea Hana
ʻO POPSO, pōkole no ka Piperazine-N,N'-bis(2-hydroxypropanesulfonic acid) sesquisodium salt, he mea hoʻopaʻa i hoʻohana pinepine ʻia i ka noiʻi biological a me biochemical. Kōkua ia i ka mālama ʻana i kahi pae pH paʻa i loko o nā hopena, ʻoi aku hoʻi i loko o ka pae pH physiological. Hoʻohana ʻia ka paʻakai PIPES sesquisodium i ka moʻomeheu cell, protein biochemistry, electrophoresis, nā ʻenehana biology molecular, nā ʻōnaehana lawe lāʻau, a me nā mea hou aku. ʻO kona hiki ke hoʻoponopono i ka pH e lilo ia i mea hana waiwai nui i nā noiʻi like ʻole a me nā noi ʻoihana.
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BES CAS:10191-18-1 Kumukūʻai Mea Hana
ʻO ka N,N-Bis(hydroxyethyl)-2-aminoethanesulfonic acid, i ʻike ʻia hoʻi ʻo BES a i ʻole N,N-Bis(2-hydroxyethyl)aminoethanesulfonic acid, he hui kemika i hoʻohana pinepine ʻia ma ke ʻano he mea hoʻopaʻa i nā noi ʻepekema a me nā ʻoihana like ʻole.
He hui zwitterionic ʻo BES, ʻo ia hoʻi, he mau uku maikaʻi a maikaʻi ʻole i loko o kona ʻano. ʻO kēia waiwai e hiki ai iā ia ke mālama pono i kahi pH paʻa i loko o nā hopena.
ʻO ka waiwai pKa o BES ma kahi o 7.4, e lilo ia i mea pono loa no ka buffering ma nā pae pH physiological. Hoʻohana pinepine ʻia ia i nā hoʻokolohua biological a me biochemical, e like me ka hoʻomaʻemaʻe protein, nā hopena enzyme, a me ka moʻomeheu cell, kahi e koʻikoʻi ai ka mālama ʻana i kahi pH kikoʻī.
Eia kekahi, hoʻohana nui ʻia ʻo BES i nā ʻano hana electrophoresis, no ka mea, kōkua ia i ka mālama ʻana i ka pH e pono ai no ka hoʻokaʻawale ʻana a me ka nānā ʻana i nā biomolecules i hoʻopiʻi ʻia, e like me nā protein a me nā waikawa nucleic.
