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TAPSO CAS:68399-81-5 Kumukūʻai Mea Hana
ʻO TAPSO (3-[N-tris(hydroxymethyl)methyl]amino]-2-hydroxypropanesulfonic acid) kahi buffer zwitterionic i hoʻohana pinepine ʻia i ka noiʻi biological a me biochemical. He mea hoʻopaʻa pono ia me kahi pKa kokoke i ka pH physiological, e kūpono ai no ka mālama ʻana i kahi pH paʻa i nā hoʻokolohua biological. Hoʻohana pinepine ʻia ʻo TAPSO i ka hoʻomaʻemaʻe protein, nā hoʻāʻo enzyme, ka moʻomeheu cell, a me nā noi electrophoresis. ʻO kona solubility wai kiʻekiʻe a me ka hoʻopilikia haʻahaʻa me nā kaʻina hana biological e lilo ia i koho kaulana i ke kaiāulu ʻepekema. Ua ʻike ʻia ʻo TAPSO no kona hopena liʻiliʻi ma ka hana enzyme a hoʻohana pinepine ʻia ma ke ʻano he koho ʻē aʻe i nā mea hoʻopaʻa ʻē aʻe e like me Tris a i ʻole nā buffers phosphate.
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6-Methyl-2-pyridinyl2-(acetylamino)-2-deoxy-1-thio-beta-D-glucopyranoside CAS:149263-94-5
ʻO 6-Methyl-2-pyridinyl 2-(acetylamino)-2-deoxy-1-thio-beta-D-glucopyranoside, i ʻike ʻia hoʻi ʻo N-acetylglucosamine thiolactone, he hui kemika me kahi ʻano kūikawā e loaʻa ana kahi apo pyridine, kahi hui acetylamino, a me kahi ʻāpana thiolactone.
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Sodium Glycocholate CAS:863-57-0
ʻO ka Sodium glycocholate kahi hui paʻakai bile e kōkua ana i ka ʻeli ʻana a me ka omo ʻana o nā momona meaʻai. Hoʻohui ʻia ia mai ka glycine a me ka cholic acid a he kuleana koʻikoʻi kona i ka wāwahi ʻana i nā momona i nā kulu liʻiliʻi, e hoʻonui ana i ko lākou ʻili no ka ʻeli pono ʻana. He mea hoʻoheheʻe loa ka Sodium glycocholate i ka wai a hoʻohana ʻia i nā ʻano lāʻau lapaʻau e hoʻonui i ka solubility a me ka bioavailability o nā lāʻau lapaʻau hiki ʻole ke hoʻoheheʻe ʻia. Eia kekahi, hoʻohana ʻia ia i nā hoʻonohonoho noiʻi a me nā hoʻokolohua hale hana e kākoʻo i ka ulu ʻana o kekahi mau bacteria a me nā ʻano cell..
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TAPS Paʻakai sodium CAS:70331-82-7
ʻO ka paʻakai sodium TAPS, i ʻike ʻia hoʻi ʻo N-(Tris(hydroxymethyl)methyl)-2-aminoethanesulfonic acid sodium salt, he mea hoʻopaʻa a me ka mea hoʻoponopono pH. Hoʻohana nui ʻia ia i ka noiʻi olaola a me ke kemika, nā lāʻau lapaʻau, a me nā diagnostics. ʻO TAPS-Na kahi hui zwitterionic e kōkua i ka mālama ʻana i kahi pH paʻa i loko o nā hopena, e kūpono ai no nā ʻano hoʻokolohua like ʻole. ʻO kāna mau noi e pili ana i ka moʻomeheu cell, ka noiʻi protein, electrophoresis, synthesis kemika, a me nā ʻano lāʻau lapaʻau. ʻO TAPS-Na kahi ʻano hiki ke hoʻoheheʻe ʻia a paʻa hoʻi o TAPS i maʻalahi ke lawelawe a hoʻohana i nā hopena wai.
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NBD chloride CAS: 10199-89-0
ʻO NBD chloride, i ʻike ʻia hoʻi ʻo 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, he kala fluorescent i hoʻohana pinepine ʻia i nā noi kemika a me nā hana olaola. No ka papa ia o nā nitrobenzoxadiazole (NBD) derivatives, e hōʻike ana i nā waiwai fluorescence ikaika.
ʻIke ʻia ʻo NBD chloride e kona hiki ke hana i nā pilina covalent me nā nucleophiles, e like me nā amines mua, thiols, a me nā hui hydroxyl. ʻAe kēia waiwai iā ia e hoʻohana ʻia ma ke ʻano he mea hōʻailona no nā biomolecules, me nā protein, peptides, nucleic acids, a me nā mole liʻiliʻi ʻē aʻe.
Ke hana ka NBD chloride me kahi nucleophile, hana ia i kahi hopena pani, e hopena ana i ka hoʻokumu ʻia ʻana o kahi huahana paʻa i hōʻailona ʻia me ka NBD. Hōʻike kēia huahana i hōʻailona ʻia i ka hoʻokuʻu fluorescence ikaika, e lilo ia i mea pono no nā ʻano hana ʻike a me ke kiʻi like ʻole.
Hiki ke hoʻonāukiuki ʻia ka hoʻokuʻu ʻana o ka fluorescence o nā molekala i hōʻailona ʻia me ka NBD me ke kukui ultraviolet (UV) ma kahi o 470 nm, a ʻike pinepine ʻia ka mālamalama i hoʻokuʻu ʻia ma kahi o 530 nm. Hāʻule kēia nalu hoʻokuʻu i loko o ka ʻāpana ʻōmaʻomaʻo o ka spectrum ʻike ʻia, kahi e haʻahaʻa ai ka hoʻopilikia ʻana o ka ʻike ʻana mai nā hāpana olaola.
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Proteinase K CAS:39450-01-6 Kumukūʻai Mea Hana
ʻO Proteinase K kahi enzyme serine protease ikaika loa e hiki ke hoʻoulu i ka hydrolysis o nā pilina peptide i loko o nā protein. Loaʻa ia mai ka fungus Engyodontium album a ua ʻike ʻia no kona laulā o ke ʻano substrate specificity. Hiki iā Proteinase K ke ʻoki pono i nā pilina peptide ma ka ʻaoʻao C-terminal o nā hydrophobic, aliphatic, a i ʻole nā waikawa amino aromatic, e lilo ia i mea pono i nā noi biology molecular a me biochemistry..
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N,N-Dimethyl-p-phenylenediamine dihydrochloride CAS:536-46-9
ʻO N,N-Dimethyl-p-phenylenediamine dihydrochloride kahi hui kemika i hoʻohana pinepine ʻia i nā noi ʻepekema a me nā ʻoihana like ʻole. He pauka crystalline keʻokeʻo a keʻokeʻo paha me ke ʻano molekala o C8H14N2·2HCl.
He ākea nā hoʻohana o kēia hui ma muli o kona mau waiwai a me ka reactivity. Hoʻohana pinepine ʻia ia ma ke ʻano he reagent analytical no ka hoʻoholo ʻana i nā peroxides a ma ke ʻano he dye intermediate i ka hana ʻana o nā kala like ʻole a me nā pigments.
Ma ke kahua o ke kemika analytical, hoʻohana pinepine ʻia ʻo N,N-Dimethyl-p-phenylenediamine dihydrochloride ma ke ʻano he hōʻailona colorimetric no ka hoʻoholo ʻana i nā ʻano oxidative, e like me ka hydrogen peroxide a i ʻole nā peroxides i loko o nā laʻana. Hoʻololi ke kala o ka hui i ka wā e hana ai me kēia mau mea oxidizing, e ʻae ana i kā lākou helu ʻana.
Eia kekahi, hiki ke loaʻa kēia hui i loko o ka hoʻohuihui ʻana o nā kala lauoho, kahi e hana ai ma ke ʻano he mea hoʻomohala kala. ʻO kona hiki ke hana i nā kala ʻōlinolino a lōʻihi e kaulana ai i ka ʻoihana hoʻonani.
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Paʻakai disodium phosphate Creatine CAS: 922-32-7
ʻO ka paʻakai Creatine phosphate disodium kahi hui i hana ʻia me ka creatine, kahi amino acid derivative kūlohelohe, i hoʻopaʻa ʻia i nā hui phosphate a me nā ion sodium. He kuleana koʻikoʻi kona i ka hāʻawi ʻana i ka ikehu no ka ʻūlū ʻana o nā ʻiʻo a hoʻohana pinepine ʻia ma ke ʻano he mea hoʻohui meaʻai e nā mea pāʻani a me nā mea hana kino.
Ke ʻai ʻia, hoʻololi ʻia ka paʻakai creatine phosphate disodium i creatine phosphate i loko o ke kino, a laila mālama ʻia i loko o nā ʻiʻo. I ka wā o nā hana ikaika, e like me ka hāpai kaumaha a i ʻole ka holo wikiwiki ʻana, hana ka creatine phosphate ma ke ʻano he kumu i loaʻa koke no nā pilina phosphate ikehu kiʻekiʻe, e ʻae ana i ka hoʻopiha hou ʻana o ka adenosine triphosphate (ATP).
ʻO ATP ka molekole ikehu mua e hoʻohana ʻia e nā ʻiʻo, a emi koke kona pae i ka wā hoʻoikaika kino ikaika. Ma ka hoʻohui ʻana me ka creatine phosphate, hiki i nā mea pāʻani ke hoʻonui i ka loaʻa ʻana o ATP, e alakaʻi ana i ka hoʻomaikaʻi ʻana i ka hana ʻiʻo, ka hoʻonui ʻana i ka mana, a me ka hoʻemi ʻana i ka luhi.
Eia kekahi, hiki i ka paʻakai creatine phosphate disodium ke loaʻa nā pono ʻē aʻe, me ka hoʻonui ʻia ʻana o ka nui o nā ʻiʻo, ka hoʻōla hou ʻana, a me ka hoʻomaikaʻi ʻana i ka hana noʻonoʻo. Lawe pinepine ʻia ia ma ka waha ma ke ʻano pauka a papa paha, ma ke ʻano he mea hoʻohui kūʻokoʻa a i ʻole ma ke ʻano he ʻāpana o kahi papa hoʻohui ma mua o ka hoʻomaʻamaʻa ʻana a i ʻole ma hope o ka hoʻomaʻamaʻa ʻana.
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2′,6′-DiMethylcarbonylphenyl-10-sulfopropylacridiniuM-9-carboxylate 4′-NHS Ester CAS:194357-64-7
ʻO 2′,6′-DiMethylcarbonylphenyl-10-sulfopropylacridinium-9-carboxylate 4′-NHS ester kahi hui kemika me kahi ʻano molekala paʻakikī. Loaʻa iā ia kahi hui sulfopropylacridinium a me kahi hui hana carboxylate ester. ʻO ke alo o ka ʻāpana ester e hōʻike ana he reactive ia a hiki ke hoʻohana ʻia ma ke ʻano he lepili a mea hoʻololi paha no nā biomolecules.
Hōʻike ka hui sulfopropylacridinium o ka hui e loaʻa paha iā ia nā noi kūpono i nā hoʻāʻo fluorescence-based, kahi hiki ke hoʻohana ʻia ma ke ʻano he probe fluorescent a i ʻole dye no ka ʻike ʻana a me ka nānā ʻana i nā biomolecules. Loaʻa paha iā ia ka pilina i nā haʻawina o nā kaʻina hana cellular, e like me ka hōʻailona calcium intracellular.
ʻO ka hoʻokomo ʻia ʻana o ka hui ester NHS e hōʻike ana hiki iā ia ke hana me nā amines mua, e like me nā mea i loaʻa i loko o nā protein a i ʻole nā peptides, e hana i nā pilina amide paʻa. ʻO kēia reactivity e lilo ia i mea pono no nā noi bioconjugation, kahi e hiki ai ke hoʻohana ʻia e lepili a hoʻololi paha i nā biomolecules me nā molekala hana ʻē aʻe, e like me nā fluorophores a i ʻole nā tags.
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ABEI CAS:66612-29-1 Kumukūʻai Mea Hana
ʻO N-(4-Aminobutyl)-N-ethylisoluminol, i ʻike ʻia hoʻi ʻo ABEI, he hui luminescent i hoʻohana pinepine ʻia ma ke ʻano he probe chemiluminescent i nā noi bioanalytical. No ka papa o nā derivatives isoluminol a hoʻohana nui ʻia i nā immunoassays a me nā ʻenehana ʻike DNA/RNA.
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Z-GLY-PRO-PNA CAS:65022-15-3
ʻO Z-GLY-PRO-PNA kahi peptide nucleic acid (PNA) i loaʻa me ke kaʻina Z-Gly-Pro. Hoʻohana pinepine ʻia kēia hui i ka noiʻi a me ka diagnostics no kona hiki ke hoʻopaʻa pono i nā kaʻina nucleic acid complementary. ʻO ka hoʻohui ʻana o ka Z-group ma ka N-terminus e ʻae ai i ka hoʻopaʻa pono ʻana a me ka lawe ʻana i nā pahuhopu molekala i makemake ʻia. Me kona hui kū hoʻokahi o nā waiwai peptide a me ka nucleic acid, hāʻawi ʻo Z-GLY-PRO-PNA i ka hiki nui i nā noi like ʻole, me ka gene therapy, molecular diagnostics, a me ka hoʻomohala ʻana i nā lāʻau lapaʻau..
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TRIS-Acetate CAS:6850-28-8 Kumukūʻai Mea Hana
ʻO TRIS-Acetate, he buffer i hoʻohana pinepine ʻia i nā hoʻokolohua biological a me biochemical. He hui pū ʻia ia o ke kumu Tris a me ka waikawa acetic, e hopena ana i kahi hopena pH-stable i hoʻohana ʻia e kāohi a mālama i ka pae pH i makemake ʻia no nā ʻano noi like ʻole. He mea pono loa ʻo TRIS-Acetate i nā haʻawina DNA a me RNA, no ka mea, hāʻawi ia i kahi kūpono no nā hana enzyme, electrophoresis, a me ka gel electrophoresis. Kōkua ia i ka mālama ʻana i ke kūpaʻa a me ka pono o nā waikawa nucleic i nā kaʻina hana like ʻole, e like me ka DNA sequencing, polymerase chain reaction (PCR), a me ka agarose gel electrophoresis. Ma waho aʻe o ka noiʻi nucleic acid, hoʻohana ʻia ʻo TRIS-Acetate i nā kaʻina hana hoʻokaʻawale protein a me ka hoʻomaʻemaʻe, ka membrane protein extraction, a me nā hoʻokolohua moʻomeheu cell. ʻO kona hiki ke buffering versatile e lilo ia i mea hana waiwai nui ma nā wahi he nui o ka noiʻi ʻepekema, e hōʻoiaʻiʻo ana i nā kūlana kūpono no nā hopena biological a me ka mālama ʻana i ke kūpaʻa o nā enzymes a me nā protein.
